The complete software suite is available for free download. Multispectral illumination can be incorporated through the use of off-the-shelf light emitting diodes which the software synchronizes to image acquisition via a programmed microcontroller, allowing arbitrary high-speed illumination sequences. The software integrates a number of modular functions through a custom graphical user interface (GUI) and provides extensive control over a wide range of inexpensive IEEE 1394 Firewire cameras. To address this issue, we have developed a novel, open-source software package to control high-speed, multispectral optical imaging systems. Despite these benefits, commercially available scientific cameras rarely include software that is suitable for in-vivo imaging applications, making this highly versatile form of optical imaging challenging and time-consuming to implement. High-speed, high resolution imaging can reveal dynamic events such as changes in blood flow and responses to stimulation.
Camera-based in-vivo optical imaging can provide detailed images of living tissue that reveal structure, function, and disease.
